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dc.contributor.authorKiliç, A. and ŞImşek, H. and Kalender, H.
dc.date.accessioned2021-04-08T12:11:08Z
dc.date.available2021-04-08T12:11:08Z
dc.date.issued2010
dc.identifier.issn13006045
dc.identifier.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-78149419427&partnerID=40&md5=893af637f03196b1fff19594a9ecb252
dc.identifier.urihttp://acikerisim.bingol.edu.tr/handle/20.500.12898/5109
dc.description.abstractAmerican foulbrood (AFB) caused by Paenibacillus larvae is a bacterial disease in honey bee larvae that is observed worldwide. The aim of this study was to detect P. larvae in honey and beeswax samples from the suspected hives by direct PCR and culture growth. AF6 and AF7, the most sensitive pair of primers, were used to identify the DNA of P. larvae. A total of 100 suspected honey and beeswax samples collected from 25 different enterprises were examined. P. larvae were identified in 7 samples out of 8 by the PCR positive in total. Eight suspected larvae of honeybees were found positive by PCR. A suspected culture-negative sample was found positive through evaluation with PCR. One beeswax sample and seven honey samples were detected as positive for the larvae in the direct PCR detection.
dc.language.isoTurkish
dc.sourceKafkas Universitesi Veteriner Fakultesi Dergisi
dc.titleDetection of american foulbrood disease (Paenibacillus larvae) by the PCR and culture [Amerikan yavru çürüklüǧü hastalıǧı etkeni (Paenibacillus larvae)'nin PCR ve kültür ile teşhisi]


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