In vitro multiple shoot regeneration from Scilla siberica subsp. armena petiole [Scilla siberica subsp. armena yaprak sapindan in vitro çoklu sürgün rejenerasyonu]

Abstract

Scilla siberica subsp. armena is a perennial blue flowered bulbous plant. This study aimed to develop an efficient multiple shoot regeneration system for S. siberica subsp. armena using petiole on MS medium containing 0.25, 0.50, 1, 2 mg L-1 TDZ and 0.1, 0.2 mg L-1 NAA (8 combinations). Maximum mean shoot regeneration percentage was noted in MS medium containing 2 mg L-1 TDZ + 0.1 mg L-1 NAA, maximum mean number of shoot per explant was recorded in MS medium containing 2 mg L-1 TDZ + 0.2 mg L-1 NAA, maximum mean number of shoot length was recorded in MS medium supplemented with 0.25 mg L-1 TDZ + 0.2 mg L-1 NAA. Well developed micro propagated shoots were rooted on MS medium containing 1 mg L-1 IBA. © 2016, Centenary University. All rights reserved.