Inhibitory Effects of Some Flavonoids on Thioredoxin Reductase Purified from Chicken Liver
Özet
Thiorecioxin reductases (TrxRs) are selenocysteine-containing
flavoenzymes that reduce Trxin NADPH-dependent manner. In the view of
the direct vital role of TrxR in a wide range of biochemical and
physiological processes, methods to inhibit this enzyme are clinically
important. TrxR has recently emerged as a new candidate in anticancer
drug investigations because of overexpression in tumorous cells. In this
study, TrxR from chick liver was purified 94.6-fold with a yield of
4.86\% and a specific activity of 0.19 EU/mg. K-M and V-max, values of
TrxR for DINB were calculated as 0.9 mM and 0,03 EU/mL, respectively.
Then, the effects of the flavonoicis hesperidin, naringenin, chlorogenic
acid, ferulic acid, naringin, 3,4-dihydoxybenzoic acid, and ellagic acid
on the enzyme activity were evaluated under in-vitro conditions. Ellagic
acid showed the strongest inhibitory activity on TrxR with a IC50 value
of 18 mu M, followed by naringenin and chlorogenic acid with IC50 values
of 46.7 mu M and 75.8 mu M, respectively. Our results showed that
flavonoids as natural products are potential inhibitors of TrxRs and the
flavonoid content in animal diets may alter metabolic pathways by
influencing TrxR activity.
Koleksiyonlar
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