Klinik Öncesi Bilimler Bölümü

Van Yöresinde Evde Beslenen Kafes Kuşlarında Bağırsak Parazitlerinin Yaygınlığının Araştırılması

2021-03-29T14:25:44Z

Van Yöresinde Evde Beslenen Kafes Kuşlarında Bağırsak Parazitlerinin Yaygınlığının Araştırılması Gül, Abdurrahman Bu araştırma, Van yöresinde evde beslenen kafes kuşlarında bulunan parazitlerin yaygınlığını araştırmak için yapılmıştır. Bunun için, 70 kafes kuşundan (6 papağan, 5 bülbül, 24 kanarya, 35 muhabbet kuşu) dışkı örnekleri alınıştır. Parazitoloji laboratuarında, 70 dışkı örneği, Helmint yumurtaları, Eimeria sp. ookistleri ve Giardia sp. kistleri yönünden nativ ve sedimentasyon, Cryptosporidium sp. ookistleri yönünden de asid-fast boyama yöntemleri ile incelenmiştir. Gaitaları incelenen 70 kafes kuşunun 6 (%8,57)’sı enfekte bulunmuştur. Eimeria sp. ookistleri, muhabbet kuşlarında (%8,57) ve kanaryada (%8,33) tespit edilirken, Cryptosporidium sp. ookistleri ise sadece papağanlarda (%16,67) tespit edilmiştir. Kafes kuşlarının hiç birinde Helmint sp. yumurtası ve Giardia sp. kisti bulunamamıştır. Bülbüllerde de hiçbir parazit tespit edilememiştir.

The first isolation of campylobacter lanienae from chickens

2021-03-29T14:25:09Z

The first isolation of campylobacter lanienae from chickens Açık, Mehmet Nuri; Karahan, Murat; Öngör, Hasan; Karagülle, Burcu; Çetinkaya, Burhan The aim of the present study was to determine the prevalence of Campylobacter jejuni, C. coli, C. lari and C. lanienae which are the most common causes of acute bacterial gastroenteritis in humans, in clinically healthy cattle, sheep and chickens. A total number of 2700 abattoir samples containing 150 intestinal contents and 150 gall bladders from each of the animal species in each of the three provinces located in the east of Turkey. Following the inoculation of the samples onto modified Charcoal Cefoperazone Deoxycholate Agar (mCCDA), Campylobacter spp. was isolated from 24.7% (667/2700). In the analysis of the isolates with speciesspecific Polymerase Chain Reaction (PCR), the identification percentages of Campylobacter species were determined as 78.1% for C. jejuni, 23.7% for C. coli, 1.2% for C. lanienae and 1.0% for C. lari. To the authors’ knowledge, this study reports the isolation of C. lanienae in animals for the first time in Turkey and in chickens species for the first time in the world.

Investigation of arcobacters in meat and faecal samples of clinically healthy cattle in Turkey

2021-03-29T14:25:01Z

Investigation of arcobacters in meat and faecal samples of clinically healthy cattle in Turkey Öngör, Hasan; Çetinkaya, Burhan; Açık, Burhan; Atabay, Halil İbrahim Aims: To investigate the presence of Arcobacter spp. in minced beef meat (n ¼ 97) and rectal faecal samples (n ¼ 200) collected from cattle immediately after slaughter at a local abattoir in Turkey. Methods and Results: Meat samples were examined using three different isolation procedures (CATsupplemented media, de Boer arcobacter isolation method and membrane filtration method), but only one method (CAT-supplemented media) was employed for faecal samples. The isolated Arcobacter strains were identified by genus- and species-(multiplex) specific PCR assays. Arcobacter spp. were isolated from 5 and 9Æ5% of meat and faecal samples respectively. Although the only Arcobacter sp. found in meat samples was Arcobacter butzleri, all three pathogenic species – A. butzleri, A. cryaerophilus and A. skirrowii – were detected in the rectal swabs. No Arcobacter was isolated when the de Boer method was used for minced meat samples but the same five meat samples were found positive for arcobacters when CAT-supplemented media and membrane filtration method were used. Conclusions: The membrane filtration method was found to be superior to the CAT-supplemented media, because it led to a reduction in competing microflora. However, the necessity for one filter and medium for each sample makes this method somewhat expensive. The multiplex-PCR (m-PCR) assay shortened significantly the time required for the identification of Arcobacter spp. and also removed the possibility of false positive results due to other campylobacteria. Significance and Impact of the Study: This study reports the isolation of Arcobacter spp. in cattle for the first time in Turkey. The m-PCR assay enables the identification and differentiation of all arcobacters simultaneously in one-step PCR.

TraT and CNF2 genes of escherichia coli isolated from milk of healthy cows and sheep

2021-03-29T14:24:48Z

TraT and CNF2 genes of escherichia coli isolated from milk of healthy cows and sheep Açık, Mehmet Nuri; Yurdakul, Erhan; Çakıcı, Leyla; Onat, Nazif; Doğan, Özlem; Çetinkaya, Burhan The objectives of the present study were to isolate Escherichia coli from milk of apparently healthy cows and sheep and to investigate the presence of traT and cytotoxic necrotising factor-2 (CNF2) virulence genes by multiplex polymerase chain reaction (PCR). Milk samples collected from a total of 1028 apparently healthy ruminants (737 cows and 291 sheep) in eastern Turkey were streaked onto 5% sheep-blood agar. E. coli was isolated and identified by biochemical tests in 5.9% (61/1028) of milk samples. Correct amplification with the molecular length of 232 bp was obtained from all E. coli isolates by the species-specific PCR. The isolation rates of the agent were calculated to be 7.6% (56/737) in cows and 1.7% (5/291) in sheep. The difference between these proportions was statistically significant (p<0.001). Multiplex PCR showed that traT and CNF2 genes were present in 62.3% and 6.6% of all isolates, respectively. Both genes were present in 16.4% of the isolates, with only 14.7% having neither gene.