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dc.contributor.authorKirici, M. and Kirici, M. and Beydemir, S. and Bülbül, M.
dc.date.accessioned2021-04-08T12:08:51Z
dc.date.available2021-04-08T12:08:51Z
dc.date.issued2016
dc.identifier.issn13071130
dc.identifier.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85011915395&partnerID=40&md5=15e460bc2a3e044837a36f6b8266e4b8
dc.identifier.urihttp://acikerisim.bingol.edu.tr/handle/20.500.12898/4700
dc.description.abstractIn this study, effects of some insecticides (cypermethrin, deltamethrin, dichlorvos, imidacloprid and lambda-cyhalothrin) on glucose 6-phosphate dehydrogenase (D-glucose-6-phosphate: NADP+ oksidoreductase, EC 1.1.1.49; G6PD) from gilthead sea bream (Sparus aurata) have been investigated in vitro. In this purpose, G6PD enzyme was purified 1864.3-fold with a yield of 54.6% from fish liver using 2',5'-ADP Sepharose 4B affinity chromatography. Activity of the enzyme was determined by Beutler method spectrophotometrically. The purify of the enzyme was checked by sodium dodecyl sulphate-polyacrylamide gel electrophoresis and the purified enzyme showed a single band on the gel. Additionally, inhibitory effects of some insecticides (cypermethrin, deltamethrin, dichlorvos, imidacloprid and lambda-cyhalothrin) on G6PD were investigated. For this aim, IC50 values for cypermethrin, deltamethrin, dichlorvos, imidacloprid and lambda-cyhalothrin were determined by plotting activity % vs. [I]. IC50 values of cypermethrin, deltamethrin, dichlorvos, imidacloprid and lambda-cyhalothrin were 0.237, 0.016, 0.071, 0.021 and 0.187 mM, respectively. From these results, we showed that deltamethrin is the most potent inhibitor of fish liver G6PD enzyme.
dc.language.isoEnglish
dc.sourceJournal of Applied Biological Sciences
dc.titleIn vitro toxicity effects of some insecticides on gilthead sea bream (Sparus aurata) liver glucose 6-phosphate dehydrogenase


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