Rejuvenation of mature lentisk by micrografting and evaluation of genetic stability
Date
2016Author
Onay, Ahmet and Tilkat, Engin and Suzerer, Veysel and Karakas Metin,
Ozge and Ozden Ciftci, Yelda and Kilinc, Fatih Mehmet and Koc, Ibrahim
and Sakiroglu, Muhammet and Yildirim, Hakan and Altinkut Uncuoglu, Ahu
and Calar, Nazan and Akdemir, Omer Faruk
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Show full item recordAbstract
An in vitro propagation method was established for both male and female
genotypes of lentisk using actively growing shoot tips derived from
forcefully lignified shoots. The effects of growth regulators on in
vitro morphogenesis were investigated. Since rooting of the regenerated
shoots for both genotypes was not achieved, an in vitro micrografting
method was developed for the production of plantlets. Moreover, genetic
stability of 3-, 6-, and 24-times subcultured clones of both genotypes
was assessed and compared with the mother plants using fluorescent-based
amplified fragment length polymorphism (AFLP) analysis. The set of main
plants and the different subcultured clones were divided into two
clusters. In the first cluster, the original male and female plants were
grouped together with the 3-times subcultured female and the 6-times
subcultured male and female groups, whereas the second cluster contained
the 24-times subcultured clones and the 3-times subcultured male group.
To the best of our knowledge, this is the first report of successfully
inducing plantlets from mature lentisk genotypes and the first analysis
of clonal fidelity of regenerated mature female and male P. lentiscus L.
plants by AFLP.
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