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dc.contributor.authorAçık, Mehmet Nuri
dc.contributor.authorYurdakul, Erhan
dc.contributor.authorÇakıcı, Leyla
dc.contributor.authorOnat, Nazif
dc.contributor.authorDoğan, Özlem
dc.contributor.authorÇetinkaya, Burhan
dc.date.accessioned2014-07-11T13:28:08Z
dc.date.available2014-07-11T13:28:08Z
dc.date.issued2004-07-04
dc.identifier.urihttp://hdl.handle.net/11472/169
dc.description.abstractThe objectives of the present study were to isolate Escherichia coli from milk of apparently healthy cows and sheep and to investigate the presence of traT and cytotoxic necrotising factor-2 (CNF2) virulence genes by multiplex polymerase chain reaction (PCR). Milk samples collected from a total of 1028 apparently healthy ruminants (737 cows and 291 sheep) in eastern Turkey were streaked onto 5% sheep-blood agar. E. coli was isolated and identified by biochemical tests in 5.9% (61/1028) of milk samples. Correct amplification with the molecular length of 232 bp was obtained from all E. coli isolates by the species-specific PCR. The isolation rates of the agent were calculated to be 7.6% (56/737) in cows and 1.7% (5/291) in sheep. The difference between these proportions was statistically significant (p<0.001). Multiplex PCR showed that traT and CNF2 genes were present in 62.3% and 6.6% of all isolates, respectively. Both genes were present in 16.4% of the isolates, with only 14.7% having neither gene.tr_TR
dc.language.isoengtr_TR
dc.publisherResearch in Veterinary Sciencetr_TR
dc.relation.isversionof10.1016/j.rvsc.2004.01.003tr_TR
dc.subjectE. colitr_TR
dc.subjecttraTtr_TR
dc.subjectCNF2tr_TR
dc.subjectMilktr_TR
dc.subjectCowtr_TR
dc.subjectSheeptr_TR
dc.titleTraT and CNF2 genes of escherichia coli isolated from milk of healthy cows and sheeptr_TR
dc.typeArticletr_TR


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