TraT and CNF2 genes of escherichia coli isolated from milk of healthy cows and sheep
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Tarih
2004-07-04Yazar
Açık, Mehmet Nuri
Yurdakul, Erhan
Çakıcı, Leyla
Onat, Nazif
Doğan, Özlem
Çetinkaya, Burhan
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The objectives of the present study were to isolate Escherichia coli from milk of apparently healthy cows and sheep and to investigate
the presence of traT and cytotoxic necrotising factor-2 (CNF2) virulence genes by multiplex polymerase chain reaction
(PCR). Milk samples collected from a total of 1028 apparently healthy ruminants (737 cows and 291 sheep) in eastern Turkey were
streaked onto 5% sheep-blood agar. E. coli was isolated and identified by biochemical tests in 5.9% (61/1028) of milk samples.
Correct amplification with the molecular length of 232 bp was obtained from all E. coli isolates by the species-specific PCR. The
isolation rates of the agent were calculated to be 7.6% (56/737) in cows and 1.7% (5/291) in sheep. The difference between these
proportions was statistically significant (p<0.001). Multiplex PCR showed that traT and CNF2 genes were present in 62.3% and
6.6% of all isolates, respectively. Both genes were present in 16.4% of the isolates, with only 14.7% having neither gene.
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http://hdl.handle.net/11472/169Koleksiyonlar
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