Total phenol content and antioxidant activity of methanolic extracts from different parts of Turkish oriental hackberry (Celtis tournefortii Lam.)

Abstract

Aim of the study: To evaluate the total phenol content and antioxidant activity of methanolic extract from different parts of Turkish oriental hackberry (Celtis tournefortii Lam.).

Material and Methods: Soxhlet assisted extraction technique was used for extraction of fruit, leaf and young twig of C. tournefortii using methanol. Antioxidant activities of the methanolic extract from different parts were evaluated by using ABTS (2,2'-azinobis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt) cation radical DPPH (2,2-diphenyl-1-picrylhydrazyl) free radical scavenging, ferric thiocyanate (FTC), reducing power and metal chelating activity assays. The total phenolic contents were determined using Folin-Ciocalteu reagent.

Results: Fruit, leaf and young twig were the valuable organs for the extractable content representing a yield of 15.24, 23.45 and 12.34% (w/w), respectively. The methanolic extracts from different parts of C. tournefortii were exhibited significant antioxidant activity against 2,2-diphenyl-1-picrylhyrazyl radical (p<0.05). According to the ferric thiocyanate assay particularly, higher activity was exhibited by leaf and young twig extracts with 96.34% and 87.65% inhibitions respectively, while 74.56% inhibition by fruit extracts. However, both fruit and leaf extracts exhibited higher reducing power activity than Vit. C. In addition, leaf extract showed highly ABTS cation radical scavenging activity than Trolox, and comparable with well known antioxidant agent BHA. The leaf extracts of C. tournefortii determined to have the highest total phenol content. Correlation analysis indicated that there was a linear relationship between the antioxidant activity and phenolic compound content of the extracts from different parts of Turkish oriental hackberry. The leaf extract of Turkish oriental hackberry can be utilized as a natural source of antioxidant.

Acknowledgements: I.H Geçibesler would like to express their sincere thanks to Mr. Mustafa Karabulut and Miss Seda Karabulut for their kind assistance to provide plant material. Author also thanks Assist. Prof. Dr. Alpaslan Koçak Department of Botanic, Science and Art Faculty, Bingol University for botanical studies.