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dc.contributor.authorŞişecioʇlu, M. and Budak, H. and Geffers, L. and Çankaya, M. and Çiftci, M. and Thaller, C. and Eichele, G. and Küfrevioʇlu, Ö.I. and Özdemir, H.
dc.date.accessioned2021-04-08T12:08:59Z
dc.date.available2021-04-08T12:08:59Z
dc.date.issued2015
dc.identifier10.1194/jlr.M059634
dc.identifier.issn00222275
dc.identifier.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-84941286978&doi=10.1194%2fjlr.M059634&partnerID=40&md5=5a994890928f88f7b325aa4d8939e3f7
dc.identifier.urihttp://acikerisim.bingol.edu.tr/handle/20.500.12898/4739
dc.description.abstractCholesterol and its biosynthetic pathway intermediates and derivatives are required for many developmental processes including membrane biogenesis, transmembrane receptor signaling, steroid biogenesis, nuclear receptor activation, and posttranslational modifi cation of hedgehog (Hh) proteins. To perform such multifaceted tasks depends on stringent regulation of expression of cholesterol biosynthetic enzymes (CBEs). We established for a whole organism, for the fi rst time, the 3D expression pattern of all genes required for cholesterol biosynthesis (CBS), starting from acetyl-CoA and ending with cholesterol. This data was produced by high-throughput in situ hybridization on serial sections through the mouse fetus. The textually annotated image data were seamlessly integrated into the METscout and GenePaint public databases. This novel information helps in the understanding of why CBEs are expressed at particular locations within the fetus. For example, strong CBE expression is detected at sites of cell proliferation and also where cell growth increases membrane surface, such as in neurons sprouting axons and forming synapses. The CBE data also sheds light on the spatial relationship of cells and tissue that express sonic Hh (Shh) and produce cholesterol, respectively. We discovered that not all cells expressing Shh are capable of CBS. This fi nding suggests novel ways by which cholesterylation of Shh is regulated.
dc.language.isoEnglish
dc.sourceJournal of Lipid Research
dc.titleA compendium of expression patterns of cholesterol biosynthetic enzymes in the mouse embryo


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