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dc.contributor.authorAkdemir, H. and Suzerer, V. and Tilkat, E. and Onay, A. and Çiftçi, Y.O.
dc.date.accessioned2021-04-08T12:08:26Z
dc.date.available2021-04-08T12:08:26Z
dc.date.issued2016
dc.identifier10.1007/s12010-016-2168-7
dc.identifier.issn02732289
dc.identifier.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-84975129428&doi=10.1007%2fs12010-016-2168-7&partnerID=40&md5=ebe9cdedd11ad45c4f9dd41c172aba49
dc.identifier.urihttp://acikerisim.bingol.edu.tr/handle/20.500.12898/4600
dc.description.abstractDetermination of genetic stability of in vitro-grown plantlets is needed for safe and large-scale production of mature trees. In this study, genetic variation of long-term micropropagated mature pistachio developed through direct shoot bud regeneration using apical buds (protocol A) and in vitro-derived leaves (protocol B) was assessed via DNA-based molecular markers. Randomly amplified polymorphic DNA (RAPD), inter-simple sequence repeat (ISSR), and amplified fragment length polymorphism (AFLP) were employed, and the obtained PIC values from RAPD (0.226), ISSR (0.220), and AFLP (0.241) showed that micropropagation of pistachio for different periods of time resulted in “reasonable polymorphism” among donor plant and its 18 clones. Mantel’s test showed a consistence polymorphism level between marker systems based on similarity matrices. In conclusion, this is the first study on occurrence of genetic variability in long-term micropropagated mature pistachio plantlets. The obtained results clearly indicated that different marker approaches used in this study are reliable for assessing tissue culture-induced variations in long-term cultured pistachio plantlets. © 2016, Springer Science+Business Media New York.
dc.language.isoEnglish
dc.sourceApplied Biochemistry and Biotechnology
dc.titleDetection of Variation in Long-Term Micropropagated Mature Pistachio via DNA-Based Molecular Markers


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