Show simple item record

dc.contributor.authorTemel, Y. and Kufrevioǧlu, Ö.I. and Çiftci, M.
dc.date.accessioned2021-04-08T12:08:17Z
dc.date.available2021-04-08T12:08:17Z
dc.date.issued2017
dc.identifier10.3906/kim-1603-135
dc.identifier.issn13000527
dc.identifier.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85014533390&doi=10.3906%2fkim-1603-135&partnerID=40&md5=8ba5b6d431ce04ae73296389814d4e33
dc.identifier.urihttp://acikerisim.bingol.edu.tr/handle/20.500.12898/4567
dc.description.abstractThe thioredoxin system, found in all living creatures, consists of thioredoxin protein (Trx), thioredoxin reductase enzyme (TrxR), and NADPH. In this study, turkey liver mitochondrial TrxR enzyme with 3.07 EU x mg-1 specific activity was purified 990-fold in a yield of 2.05% using 2′,5′-ADP Sepharose 4B affinity chromatography. The purity of the enzyme was measured and the molecular weight of its subunits was determined to be 45.5 kDa by SDS-PAGE. The molecular mass of the enzyme's natural state was found to be 88 kDa using Sephadex-G 150 gel filtration chromatography. In addition, characteristic and kinetic properties of the enzyme were determined. Then the inhibitory effects of some heavy metal ions (Ag+, Fe3+, Cd2+, Cu2+, Zn2+, Pb2+, Ni2+, and Co2+) on the activity of TrxR enzyme were examined under in vitro conditions. IC50 values were found with the heavy metal concentration with which 50% of the activity of the TrxR enzyme was inhibited. Finally, Ki values for these substances were calculated from the Lineweaver-Burk plots. It was determined that Ag+, Fe3+, Cd2+, Cu2+, and Zn2+ ions inhibited TrxR enzyme, Pb2+ ion increased enzyme activity, and Ni2+ and Co2+ ions had no effect on enzyme activity. © TÜBİTAK.
dc.language.isoEnglish
dc.sourceTurkish Journal of Chemistry
dc.titleInvestigation of the effects of purification and characterization of turkey (Meleagris gallopavo) liver mitochondrial thioredoxin reductase enzyme and some metal ions on enzyme activity


Files in this item

FilesSizeFormatView

There are no files associated with this item.

This item appears in the following Collection(s)

Show simple item record