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dc.contributor.authorTemel, Y. and Kocyigit, U.M.
dc.date.accessioned2021-04-08T12:07:53Z
dc.date.available2021-04-08T12:07:53Z
dc.date.issued2017
dc.identifier10.1002/jbt.21927
dc.identifier.issn10956670
dc.identifier.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85019736517&doi=10.1002%2fjbt.21927&partnerID=40&md5=b07835c3cdf243d7d0599d3c182ecaf0
dc.identifier.urihttp://acikerisim.bingol.edu.tr/handle/20.500.12898/4465
dc.description.abstractGlucose-6-phosphate dehydrogenase (G6PD) is the first enzyme on which the pentose phosphate pathway was checked. In this study, purification of a G6PD enzyme was carried out by using rat erythrocytes with a specific activity of 13.7 EU/mg and a yield of 67.7 and 155.6-fold by using 2′,5′-ADP Sepharose-4B affinity column chromatography. For the purpose of identifying the purity of enzyme and molecular mass of the subunit, a sodium dodecyl sulfate-polyacrylamide gel electrophoresis was carried out. The molecular mass of subunit was calculated 56.5 kDa approximately. Then, an investigation was carried out regarding the inhibitory effects caused by various metal ions (Fe2+, Pb2+, Cd2+, Ag+, and Zn2+) on G6PD enzyme activities, as per Beutler method at 340 nm under in vitro conditions. Lineweaver–Burk diagrams were used for estimation of the IC50 and Ki values for the metals. Ki values for Pb+2, Cd+2, Ag+, and Zn+2 were 113.3, 215.2, 19.4, and 474.7 μM, respectively. © 2017 Wiley Periodicals, Inc.
dc.language.isoEnglish
dc.sourceJournal of Biochemical and Molecular Toxicology
dc.titlePurification of glucose-6-phosphate dehydrogenase from rat (Rattus norvegicus) erythrocytes and inhibition effects of some metal ions on enzyme activity


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