dc.contributor.author | Agca, C.A. and Kırıcı, M. and Nedzvetsky, V.S. and Gundogdu, R. and Tykhomyrov, A.A. | |
dc.date.accessioned | 2021-04-08T12:06:18Z | |
dc.date.available | 2021-04-08T12:06:18Z | |
dc.date.issued | 2020 | |
dc.identifier | 10.1002/cbdv.202000441 | |
dc.identifier.issn | 16121872 | |
dc.identifier.uri | https://www.scopus.com/inward/record.uri?eid=2-s2.0-85090091992&doi=10.1002%2fcbdv.202000441&partnerID=40&md5=c7c5684b1357093dc28c70d380275559 | |
dc.identifier.uri | http://acikerisim.bingol.edu.tr/handle/20.500.12898/3875 | |
dc.description.abstract | Resistance to chemotherapeutic drugs is a critical problem in cancer therapy, but the underlying mechanism has not been fully elucidated. TP53-induced glycolysis regulatory phosphatase (TIGAR), an important glycolysis and apoptosis regulator, plays a crucial role in cancer cell survival by protecting cells against oxidative stress-induced apoptosis. In the present study, we investigated whether TIGAR is involved in epithelial-mesenchymal transition (EMT) in doxorubicin (DOX)-resistant human non-small cell lung cancer (NSCLC), A549/DOX cells. We found that the expression of TIGAR was significantly higher in A549/DOX cells than in the parent A549 cell lines. siRNA-mediated TIGAR knockdown reduced migration, viability and colony survival of doxorubicin-resistant lung cancer cells. Also, TIGAR knockdown decreased pro-survival protein Bcl-2 and increased pro-apoptotic Bax and cleaved poly (ADP-ribose) polymerase (PARP). Moreover, TIGAR depletion significantly up-regulated both caspase-3 and caspase-9 expression. Furthermore, TIGAR depletion up-regulated the expression of E-cadherin and down-regulated the expression of vimentin. These results indicate that TIGAR knockdown may inhibit EMT in doxorubicin (DOX)-resistant human NSCLC and may represent a therapeutic target for a non-small lung cancer cells chemoresistance. © 2020 Wiley-VHCA AG, Zurich, Switzerland | |
dc.language.iso | English | |
dc.source | Chemistry and Biodiversity | |
dc.title | The Effect of TIGAR Knockdown on Apoptotic and Epithelial-Mesenchymal Markers Expression in Doxorubicin-Resistant Non-Small Cell Lung Cancer A549 Cell Lines | |