Epidemiology, etiology and gene analysis of spike S protein of porcine epidemic diarrhea virus infection in Ukraine during 2016-2017
Date
2017Author
Masiuk, D. M. and Sosnitsky, O. I. and Nedzvetsky, V. S. and Kokarev, A.
V. and Koliada, S. G.
Metadata
Show full item recordAbstract
The spread of PEDV of pigs continues in various countries and regions of
the world. PED was first registered in several areas of Ukraine in 2014.
At present, there is a limited number of publications on the
characteristics of the spread of the virus infection in Ukraine.
Moreover, obtained research data do not clarify the epizootic situation
as a whole. Having regard to the state of pig farm industry and
permanent growth of losses caused with PEDV infection, the study of PED
in Ukraine is relevant and important. Therefore, the goal of the work
was to clarify the epizootic situation of PED in various areas of
Ukraine. We studied the epizootic state of PED with a typical course in
pig farms and the made a primary assessment of the relation of genetic
groups between identified pathogens from distinct regions of the country
where the highest intensity of the epizootic process was determined. The
work was carried out in the Biosafety Center (Ukraine). To determine the
epizootic situation of PED during 2016-2017 we collected samples of the
blood serum and biological materials from pigs from 105 agricultural
enterprises that are located in 85 administrative regions of Ukraine. In
total we tested 543 serum samples and 591 samples of pathological
tissues. The detection of PEDV was performed by PCR-RT. Serological
diagnostics were tested with ELISA. Sequence analysis was performed
according to Sanger. The results of sequences were analyzed in the
FinchTV / Chromas program. The analysis of the epizootic features of PED
in different areas of Ukraine for 2016-2017 established that PEDV is the
main causative agent of the infection. An infection circulates among
sensitive animals in pig farms and induces PED, which is difficult to
control and weakly managed as a particularly dangerous emergent
infectious disease of pigs. In 2016, 6.5\% of administrative districts
located in 12 of the 25 regions of Ukraine were included in the study,
and in 2017, 10.8\% of administrative districts located in the 14
regions of the 25 regions of the country were investigated. In general,
during 2016 and 2017 1134 samples of blood serum and animal tissue were
collected from 105 agricultural enterprises from 17.3\% of the
administrative regions of Ukraine. The obtained results showed that in
2016 PED was registered in 7 administrative districts from 4 regions of
Ukraine, and in 2017 the infection was registered in 2 regions. The
results of RNA sequence analysis of the PED virus identified the
characteristics of the PEDV genes which reflect a high degree of
homology with the Chinese strain BJ-2011-1. The analysis of the obtained
results revealled the relation of PEDV circulating in Ukraine to the
strains identified in the USA. Moreover, the samples of PEDV from
Ukrainian pig farms have the sequences that belong to the North American
clade II from the group of 2 strains of the PED virus. However, the PEDV
observed in Ukraine is different from them by at least one nucleotide
replacement of the gene which codes a protein of the S domain. Based on
the obtained results, it can be assumed that in Ukraine the PED virus
could have spread from East Asia.
Collections
![Creative Commons License](http://i.creativecommons.org/l/by-nc-nd/4.0/88x31.png)
DSpace@BİNGÖL by Bingöl University Institutional Repository is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 Unported License..