The role of inducible nitric oxide synthase in urinary bladders of cattle with enzootic hematuria and its struggle with uroplakin III
Tarih
2018Yazar
Keles, Hikmet and Bozkurt, Mehmet Fatih and Ulucan, Aykut and Demirel,
Hasan Huseyin and Yuksel, Hayati and Karadas, Erkan and Ozkul, Ayhan
Üst veri
Tüm öğe kaydını gösterÖzet
Changes in uroplakin secretion in the urothelium leads a gradual ability
lost in neoplastic conditions. Urothelium is one of the target regions
where nitric oxide can damage. Therefore, in this study, the presence of
iNOS and UPIII were investigated in bovine enzootic hematuria cases. The
results were supported by comparing them with those achieved by critical
markers for malignancy of tumor such as caspase 3, Bax, p53, PCNA, and
VEGF. The presence of 8-OHDG, a good marker for oxidative DNA damage,
was also investigated. For this purpose, urinary bladders with enzootic
hematuria were collected from the slaughterhouses. In histopathological
examinations, neoplastic, pre-neoplastic, and inflammatory lesions were
observed in these urinary bladders. In immunohistochemical examinations,
iNOS reacts as negative in the UPIII positive healthy urothelium which
preserves the structure of AUM. On the other hand positive reaction has
been observed in AUM modified and UPIII negative urothelium. In these
cases, as an indicator of oxidative damage, 8-OHdG positivity was
observed in the UPIII negative and iNOS positive areas. Additionally,
increased cell proliferation, decreased apoptosis as well as p53
disorganization were clearly observed in most of the cases. Thus, the
active role of nitric oxide, as a free radical derivative, has been
shown in the enzootic hematuria cases of cattle.
Koleksiyonlar
![Creative Commons License](http://i.creativecommons.org/l/by-nc-nd/4.0/88x31.png)
DSpace@BİNGÖL by Bingöl University Institutional Repository is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 Unported License..