The effect of graft application and allopurinol treatment on calvarial bone defect in rats
Date
2019Author
Lacin, Nihat and Izol, Bozan Serhat and Ozkorkmaz, Ebru Gokalp and
Deveci, Busra and Tuncer, Mehmet Cudi
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Show full item recordAbstract
Purpose: To investigate the effects of allopurinol administration on
osteoinductive reaction and bone development with graft material.
Methods: Thirty-six Wistar albino rats were divided into 3 groups. In
the control group, calvarial bone defect was only created without any
treatment. In the Defect + Graft group, allograft treatment was
performed by forming 8 mm calvarial bone defect. In the Defect + Graft +
Allopurinol group, alloplastic bone graft was placed in the calvarial
bone defect and then, allopurinol (50 mg/kg/day) treatment was
intraperitoneally applied for 28 days.
Results: Histopathological examination revealed inflammation, congestion
in the vessels, and an increase in osteoclast cells in the defect area.
We also observed that new osteocyte cells, increase in connective tissue
fibers, and new bone trabeculae. Osteopontin expression was positive in
osteoblast cells and lacunated osteocyte cells were located in the
periphery of the new bone trabeculae. Osteopontin expression was also
positive in osteoblasts and osteocytes cells of new bone trabeculae in
the graft site.
Conclusion: It has been shown that allopurinol treatment in rat calvaria
defects may induce osteoblastic activity, matrix development, mature
bone cell formation and new bone formation when used with autogenous
grafts.
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