The Effects of UV Irradiation and Incubation Time on in vitro Phenolic Compound Production in `Karaerik' Grape Cultivar
Tarih
2020Yazar
Celik, Mustafa and Keskin, Nurhan and Ozdemir, Fethi Ahmet
Üst veri
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In this study, the effect of Ultraviolet (UV) irradiation on induction
of individual and total phenolics production on callus cultures of
`Karaerik' grape cultivar was investigated. Callus tissues were obtained
from the leaves of the cuttings grown in in vitro plants. As a culture
medium, Gamborg B-5 was utilized with 0.1 mg L-1 NAA (Naphthaleneacetic
acid) and 0.2 mg L-1 Kin (Kinetin). Callus tissues were sub-cultured
twice with 21 days intervals. After the second subculture, 12-day-old
callus tissues were exposed to 254 nm UV-C light at 10 cm distance from
the source for 10 and 15 min by opening covers of the petri dishes in
sterile cabin. After the treatment, callus tissues were incubated under
dark conditions. Phenolic compounds were measured at 24th, 48th and 72nd
hours. Individual phenolic compounds were analyzed by HPLC (High
Pressure Liquid Chromatography) and total phenolic compounds were
measured by spectrophotometer. As a result of the study, it was found
that UV irradiation was effective for induction the production of
phenolic compounds in the callus tissues of `Karaerik' grape cultivar
and this effect was closely related to the application time.
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